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Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)
Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| Catalog # | H1830-52 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilutions | Western Blot: 0.01-0.5ug/ml detects phosphorylated HSP27 in 20ug of a cell lysate prepared from MCF-7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200mM arsenite for 30 minutes. Untreated and heat shock, and arsenite treated MCF-7 cell lysate were resolved by electrophoresis, transferred to nitrocellulose and probed with 0.05ug/ml of H1830-52. Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. | | Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 2T7 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa73-83 (YSRAL[pS]RQLSS-C) of human HSP27 with a C-terminus cysteine added for conjugation, conjugated to KLH. | | Specificity | Recognizes human phosphorylated HSP27 (Ser78). Does not recognize the non-phosphorylated form of HSP27. An additional band was observed at ~54kD in longer exposures. Species Sequence Homology: canine, 100%; Mouse and rat based on GenPept database sequence where Ser78 is replaced with Asp. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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