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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)

Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Catalog #H1830-52
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.01-0.5ug/ml detects phosphorylated HSP27 in 20ug of a cell lysate prepared from MCF-7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200mM arsenite for 30 minutes. Untreated and heat shock, and arsenite treated MCF-7 cell lysate were resolved by electrophoresis, transferred to nitrocellulose and probed with 0.05ug/ml of H1830-52. Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended secondary antibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No2T7
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa73-83 (YSRAL[pS]RQLSS-C) of human HSP27 with a C-terminus cysteine added for conjugation, conjugated to KLH.
SpecificityRecognizes human phosphorylated HSP27 (Ser78). Does not recognize the non-phosphorylated form of HSP27. An additional band was observed at ~54kD in longer exposures. Species Sequence Homology: canine, 100%; Mouse and rat based on GenPept database sequence where Ser78 is replaced with Asp.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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