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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -HSP27 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein, HSPB1, HSP28)

Anti -HSP27 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27,
Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock
Protein, HSPB1, HSP28)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
HSP27, also known as Heat shock 27kD protein, is a cytoplasmic protein in interphase cells and colocalizes with mitotic spindles in mitotic cells. It translocates to the nucleus during heat shock. HSP27 belongs to the small heat shock protein (HSP20) family and is known to be an important protein in stress resistance and actin organization and associates with alpha- and beta-tubulin, microtubules and CRYAB. It also interacts with other heat shock proteins like HSPB8 and HSPBAP1. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells. Ubiquitous expression is seen in most tissues with highest levels in heart and in tissues composed of striated and smooth muscle. Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuronopathy type 2B (HMN2B).
Catalog #H1830-52N
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman Skeletal Muscle lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa50-100 of human HSP27.
SpecificityRecognizes human HSP27. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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