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You are here:Home » Antibodies » Antibodies-Heat Shock Proteins » Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27) (BSA & Azide Free)

Anti -Heat Shock Protein 27, phosphorylated (Ser78) (HSP27) (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors.
Catalog #H1830-52X
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.01-0.5ug/ml detects phosphorylated HSP27 in 20ug of a cell lysate prepared from MCF-7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200mM arsenite for 30 minutes. Untreated and heat shock, and arsenite treated MCF-7 cell lysate were resolved by electrophoresis, transferred to nitrocellulose and probed with 0.05ug/ml of H1830-52X. Proteins were visualized using IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2T7
FormSupplied as a liquid in PBS. No preservative added. No stabilizing proteins added.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa73-83 (YSRAL[pS]RQLSS-C) of human HSP27 with a C-terminus cysteine added for conjugation, conjugated to KLH.
SpecificityRecognizes phosphorylated human HSP27 (Ser78). Does not recognize the non-phosphorylated form of HSP27. An additional band was observed at ~54kD in longer exposures. Species sequence homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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