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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 27 (HSP27)

Anti -Heat Shock Protein 27 (HSP27)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Hsp27 was recently found to be identical to the estrogen induced p29" and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. Hsp27 has also been implicated in drug resistance in cancer cells.
Catalog #H1830-53
ApplicationsSuitable for use in Western Blot and Immunohistochemistry (frozen & formalin/paraffin). Other applications have not been tested.
Recommended DilutionWestern Blot:12ug/ml for 2 hours at RT.
Immunohistochemistry: 12ug/ml for 30 minutes at RT.
Note: Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 1020 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Antigen DistributionHsp27 is identical to estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are hsp27 positive, especially those that are positive for ER and/or PgR. Hsp 27 is also complicated in drug resistance.
Positive ControlBT474 or MDA-MB-453 cells or breast carcinoma.
Cellular LocalizationCytoplasmic / nuclear.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q2324
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormLiquid containing 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
PurityPurified immunoglobulin
ImmunogenPartially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
SpecificityRecognizes an estrogen-regulated protein of 2427kD which is identified as hsp27. Species Crossreactivity: BT474 or MDA-MB-453 cells or breast carcinoma.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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