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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 27 (HSP27, Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein)

Anti -Heat Shock Protein 27 (HSP27, Heat Shock Protein beta-1, HspB1, Heat Shock 27kD
Protein, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat
Shock Protein)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Catalog #H1830-53B
Cellular Localization Hsp27 is cytoplasmic in interphase cells, localising to the nucleus under heat shock conditions. Hsp27 localises to mitotic spindle microtubules.
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormPBS, 1mg/ml BSA
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide: LLRGPSWDPFRC, corresponding to amino acids 10-21 of Human Hsp27. This peptide is 81% conserved in mice, rats, and hamsters.
SpecificitySpecies Crossreactivity: Crossreacts with Human and Rat. Expected to crossreact with canine (90% identity with immunogen) due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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