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You are here:Home » Antibodies » Antibodies-Heat Shock Proteins » Anti -Heat Shock Protein 27 (HSP27, Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein)

Anti -Heat Shock Protein 27 (HSP27, Heat Shock Protein beta-1, HspB1, Heat Shock 27kD
Protein, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat
Shock Protein)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC IF
Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to>30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence,
with an a-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein
monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5).
HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second essenger-mediated
signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo- tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase- 9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and
with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).
Catalog #H1830-53G
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence:1:200
Western Blot:1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No10F714
FormSupplied as a liquid in PBS in 0.09% sodium azide and 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to human Hsp 25/27.
SpecificityRecognizes hsp25 and hsp27, and cross-reacts with alpha B crystallin. Species Crossreactivity: rat, bovine, canine, guinea pig, hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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