Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 32 (Heme Oxygenase-1, HO-1)

Anti -Heat Shock Protein 32 (Heme Oxygenase-1, HO-1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B IH
Heme oxygenase-1 was first purified from rat liver and porcine spleen and shown to have a molecular weight of 32kD. Increased expression of HO-1 can be induced by a large number of structurally unrelated pharmacological and other agents, as well as heat shock and other forms of cellular stress. Baseline levels of HO-1 expression are high in certain tissues, including spleen and liver, but enriched enzyme preparations are recommended for experimental analysis.
Catalog #H1830-60
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applicaitons have not been tested.
Recommended DilutionsWestern Blot: 1:1000 (using ECL). A single band of ~32kD is observed. Reactivity can be completely abolished by pre-adsorption of the antibody with the complementary peptide (See cat. #H1830-61).
Immunohistochemistry: 1:50-1:1000 on human airway biopsy, human spleen, human kidney and porcine arteries.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01M sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to aa12-25 + Cys-NH2, HDLSEALKEATKEVHC-NH2 of human Heat Shock Protein 32.
SpecificityRecognizes human Heat Shock Protein 32 (Heme Oxygenase-1, HO-1). Species Crossreactivity: rat spleen, liver and testis, Madin-Darby bovine (MDBK) kidney cells and mouse RAW 264.7 cells following stimulation with gamma interferon and lipopolysaccharide. Does not react with canine (MDCK) kidney cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links