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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH IF

Heat shock proteins are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. Eukaryotic cells contain an HSP70 multigene that differ in their intracellular location and regulation. Members of the HSP70 family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes, as well as in the immune response. HSP72 was found to increase dramatically in the brains of Alzheimer?s disease patients, and was localized exclusively in neuritic plaques and neurofibrillary tangles. HSP70 concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed.
Catalog #	H1830-94G
Applications	Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunofluorescence: Immunofluorescent staining demonstrates a rapid and reversible accumulation of HSP70 within the nucleus of heat-stressed (42ºC, 1 hour) human fibroblasts.
	Western Blot: 1:5000
	Immunohistochemistry: Frozen
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8L126
Host	Mouse
Source	Bovine
Concentration	Not Determined
Form	Supplied as a liquid, 15mM sodium azide.
Purity	Ascites
Immunogen	Full length native purified Heat Shock Protein 70 from bovine brain.
Cellular Localization	Cytoplasmic and nuclear
Specificity	Recognizes bovine Heat Shock Protein 70 (HSP70) from brain and other tissues. In Western Blot, localizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species Crossreactivity: rat, chicken, human, Drosophila melanogaster, guinea pig, hamster, plants, rabbit, C. elegans.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.