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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Cognate Protein 70, cytosolic (HSC70, HSP73)

Anti -Heat Shock Cognate Protein 70, cytosolic (HSC70, HSP73)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Cytosolic Hsc70 is a constitutively expressed member of the multigene 70kD family of stress proteins. During cold acclimation, spinach cytosolic protein levels remain constant whereas mRNA levels increase (2). This low-temperature induced up-regulation of mRNA may be needed to maintain the steady state level of cytosolic Hsc70 protein (2). Spinach cytosolic Hsc70 mRNA levels have also been shown to increase in response to heat shock, but remain unchanged during drought stress (2). This is in contrast to spinach BiP (another constitutively expressed member of the 70kD family of stress proteins that resides in the endoplasmic reticulum) mRNA levels which decrease in response to heat and drought stress (2). These findings would suggest that differentially induced mRNA levels of cytosolic Hsc70 and BiP may be important in maintaining proper protein biogenesis when faced with environmental stresses (2).
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Catalog #H1831-25C
Recommended DilutionWestern Blot (Colorimetric): 1ug/mL
Immunoprecipitation: 20ug/ml
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No4E112
HostMouse
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenA preparation of spinach Hsc70 proteins comprised of cytosolic Hsc70, ER luminal Hsc70 (also known as BiP) and possibly other members of the Hsc70 family of proteins.
SpecificityRecognizes plant cytosolic Heat Shock Cognate Protein 70 at ~70-75kD. Reacts with samples from apple, asparagus, banana, carrot, corn, cucumber, lettuce, orange, potato, spinach, tomato, woody plant bark tissue and Douglas Fir root. In some plant lysates, additional lower molecular mass bands may be detected, which correspond to either Hsc70 degradation or proteins of unknown identity. Not recommended for immunoprecipitation with tomato lysates. Does not cross react with E. coli DnaK, mammalian Hsc70, recombinant hamster BiP or spinach BiP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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