Anti -HSPCB (Heat Shock 84kD, HSP 84, Heat Shock Protein HSP 90-beta, HSP 90, HSP90AB1,
HSP90B, HSPC2, HSP84)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IH IF |
|
| Cells respond to heat and chemical stress by inducing the synthesis of a group of proteins referred to as heat shock proteins. HSP90 is present in cells at high levels in the cytoplasm and is composed of two separate gene products, HSP90a and HSP90b. | | | Catalog # | H1832-98H | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 5-10ug/ml for 2hrs at RT | | Immunoprecipitation: 10ug/mg protein lysate | | Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT | | Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10min followed by cooling at RT for 20min | | Optimal dilutions to be determined by the researcher. | | Positive Control | MAD109 cells, breast carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG2a | | Host | Rabbit | | Source | Mouse | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to the N- terminus of aa2-13 mouse heat shock protein 90b. | | Specificity | Recognizes mouse Heat Shock Protein 90b. Species Crossreactivity: human and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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