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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 105 (HSP105, HSPH1)

Anti -Heat Shock Protein 105 (HSP105, HSPH1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1), also known as HSP105 and HSP110, exists as two isoforms, alpha and beta. HSPH1 alpha consists of 858 amino acids whereas HSPH1 beta has 814 amino acids. Alpha and beta have calculated molecular weights of 96.4 kD and 92 kD, respectively, but an apparent molecular weight of 105-110 kD in SDS-PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. HSPH1 alpha and beta isoforms share 94% identity. Rat HSPH1 has 92% and 97% identity to human and mouse HSPH1, respectively.
Applications:
Western Blot: An antibody concentration of 0.1ug/ml is recommended.
Optimal dilutions should be determined by the individual laboratory.
Catalog #H1833-02
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.1ml is used, the concentration will be 1mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant rat HSPH1 (rrHSPH1; aa595-733)
SpecificityDetects endogenous human, mouse, and rat HSPH1 in Western Blots. Predicted to react with HSPH1 alpha and beta isoforms based on sequence identity of the immunogen.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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