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You are here:Home » Antibodies » Antibodies-Heat Shock Proteins » Anti -HSPH1 (Heat Shock Protein 105kD, Antigen NY-CO-25, Heat Shock 110kD Protein, HSP105, HSP110, KIAA0201)

Anti -HSPH1 (Heat Shock Protein 105kD, Antigen NY-CO-25, Heat Shock 110kD Protein, HSP105,
HSP110, KIAA0201)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1, also known as HSP105 and HSP110), exists as three isoforms. HSPH1alpha is 858aa in length and is constitutively expressed. HSPH1beta is 814aa and is induced by heat shock or cell stress. HSPH1 isoform 3 is 817aa and has not been experimentally confirmed. The HSPH1 isoforms have calculated molecular weights of 92-96kD but run at an apparent molecular weight of 105-110kD in SDS-PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. Over aa human 388-505, human HSPH1 has 98% and 96% identity to mouse and rat HSPH1, respectively.
Catalog #H1833-02D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:0.5ug/ml
Optimal dilutions to be determined by the researcher.
HybridomaElicited from a mouse immunized with purified, E. coli-derived recombinant Heat shock protein H1.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No10K65
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute in 100ul of PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified, E. Coli-derived recombinant heat shock protein H1. (HSPH1; aa388-505; Accession #Q92598).
SpecificityRecognizes human HSPH1. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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