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H1833-02D Mouse Anti-HSPH1 (Heat Shock Protein 105kD, Antigen NY-CO-25, Heat Shock 110kD Protein, HSP105, HSP110, KIAA0201)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
Q92598
Isotype
IgG1
Clone Number
10K65
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1, also known as HSP105 and HSP110), exists as three isoforms. HSPH1alpha is 858aa in length and is constitutively expressed. HSPH1beta is 814aa and is induced by heat shock or cell stress. HSPH1 isoform 3 is 817aa and has not been experimentally confirmed. The HSPH1 isoforms have calculated molecular weights of 92-96kD but run at an apparent molecular weight of 105-110kD in SDS-PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. Over aa human 388-505, human HSPH1 has 98% and 96% identity to mouse and rat HSPH1, respectively.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot:0.5ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
Elicited from a mouse immunized with purified, E. coli-derived recombinant Heat shock protein H1.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified, E. Coli-derived recombinant heat shock protein H1. (HSPH1; aa388-505; Accession #Q92598).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human HSPH1. Species Crossreactivity: mouse, rat.
Conjugates
Pricing
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