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You are here:Home » Antibodies » Antibodies-Heat Shock Proteins » Anti -Heat Shock Protein 25 (HSP25)

Anti -Heat Shock Protein 25 (HSP25)


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Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IH IC
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the “small heat shock proteins” is comprised of a diverse group of proteins from ~15 to >30kD, with a single form found in yeast and human cells and multiple related forms found in higher plants. A direct relationship between elevated stress protein expression and neoplasia has been suggested from studies on estrogen-induced proteins in breast cancer cells. In addition, Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) & calcium ionophores (4). The expression of a 24kD protein was shown to correlate with steroid hormone receptors in cell lines, organs , and tumors (5, 6). The 24kD estrogen-induced protein was found to be expressed at high levels in carcinoma of the breast. Another study has suggested that the levels of Hsp27 correlate with endometrial adenocarcinomas (7). Interestingly, the regulation of human Hsp27 by estrogens and heat shock is reminiscent of the ecdysteroid and heat shock activation of the Drosophila Hsp20 genes (8).
Catalog #H1834-01
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot (Colorimetric): 1ug/ml
Immunoprecipitation: 1:50
Immunohistochemistry: 1:100-1:500
Immunocytochemistry: 1:50
Optimal dilutions to be determined by researcher.
Positive ControlsRecombinant Murine Hsp25 Protein
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a serum, 40% glycerol.
ImmunogenRecombinant mouse Hsp25
SpecificityRecognizes a 25kD protein, corresponding to the apparent molecular mass of Hsp25 on SDS-PAGE immunoblots,Species Crossreactivity: mouse, rat, hamster, guinea pig, bovine and canine. Reacts with both the phosphorylated and the non-phosphorylated forms of hsp25. We recommend Hsp27 antibodies for use in human and monkey samples.
NoteIt has been reported that certain murine cell lines do not express Hsp25 under certain conditions. Also recognizes a mitochondrial small Hsp (~35kD) in PC12 cells. This mitochondrial small Hsp is proposed to protect mitochondrial complex I from oxidative stress.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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