| Human Hsp27, mouse Hsp25 and alpha-beta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8–40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur (1). The state of phosphorylation and oligomerization of Hsp27 has been suggested to regulate microfilament organization because data demonstrates that only the non-phosphorylated lower molecular weight forms of Hsp27 bind actin barbed ends and inhibit polymerization (2). Hsp27 is also believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27 (3). Recent findings indicate a novel function for Hsp27 which is that Hsp27 is a negative regulator of cytochrome c-dependent activation of procaspase-3. (4). |
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| Catalog # | H1834-05A |
| Applications | Suitable for use in ELISA, Western Blot and Immunofuorescence. Other applications not tested. |
| Recommended Dilutions | Western: Blot (Colorimetric): 1ug/mL |
| Immunoprecipitation: 12.5ug/mL |
| Immunohistochemistry: 5ug/mL |
| Immunocytochemistry: 10ug/mL |
| Immunoelectron Microscopy: 1:50 Optimal dilutions to be determined by researcher. |
| Positive Controls | HeLa Heat Shocked Cell Lysate and Recombinant Human Hsp27 Protein |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 4E116 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Purified Hsp24 isolated from MCF-7, a human breast tumor cell line (7). Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27 |
| Specificity | Detects a ~27kD protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots. Species crossreactivity: human, monkey and fish (rainbow trout). It does not exhibit reactivity with rodent Hsp25, alphaA, alphaB or beta-crystallin proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
