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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 27 (HSP27) (Biotin)

Anti -Heat Shock Protein 27 (HSP27) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC
Human HSP27, mouse HSP25 and alpha-beta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. HSP27 is believed to exist mainly as oligomers of as many as 840 HSP27 protein monomers. Data suggests that the large oligomers of HSP27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and HSP70-dependent refolding can occur (1). The state of phosphorylation and oligomerization of HSP27 may regulate microfilament organization; data demonstrates that only the non-phosphorylated lower molecular weight forms of HSP27 bind actin barbed ends and inhibit polymerization (2). HSP27 may also protect cells by enhancing cellular glutathione levels. Wild-type HSP27 and mutant forms have shown that cellular glutathione levels depend on the oligomerization of HSP27 (3). Recent findings indicate that HSP27 is a negative regulator of cytochrome c-dependent activation of procaspase-3 (4).
Catalog #H1834-05C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunoelectron Microscopy, Apoptosis Induction, and EIA/ELISA. Other applications not tested.
Recommended DilutionWestern Blot (ECL): 1ug/ml
Immunohistochemistry: 5ug/ml
immunocytochemistry: 10ug/ml
Immunoelectron Microscopy: 1:50
Optimal dilutions to be determined by the researcher.
Positive ControlH1838-01: HeLa Heat Shocked Cell Lysate,
H1830-57: Recombinant Human Hsp27 Protein
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4E117
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, and 0.09% sodium azide before the addition of glycerol to 40%. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified HSP24 isolated from MCF-7, a human breast tumor cell line. Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, HSP27.
SpecificityRecognizes a ~27kD protein, corresponding to the apparent molecular mass of HSP27 on SDS-PAGE immunoblots.
Species CrossreactivityHuman, monkey and fish (rainbow trout). Does not exhibit reactivity with rodent HSP25, alphaA, alphaB or beta-crystallin proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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