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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 27 (HSP27) (FITC)

Anti -Heat Shock Protein 27 (HSP27) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified FC
Human Hsp27, mouse Hsp25 and alpha-beta-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 840 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur (1). The state of phosphorylation and oligomerization of Hsp27 has been suggested to regulate microfilament organization because data demonstrates that only the non-phosphorylated lower molecular weight forms of Hsp27 bind actin barbed ends and inhibit polymerization (2). Hsp27 is also believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27 (3). Recent findings indicate a novel function for Hsp27 which is that Hsp27 is a negative regulator of cytochrome c-dependent activation of procaspase-3. (4).
Catalog #H1834-05D
ApplicationsSuitable for use in FACS. Other applications have not been tested.
Recommended DilutionFACS: 10ug/ul
Optimal dilutions to be determined by researcher.
Positive Controls:
Recombinant Human Hsp27 Protein
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4E118
HostMouse
SourceHuman
Concentration0.65mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified immunoglobulin
ImmunogenPurified Hsp24 isolated from MCF-7, a human breast tumor cell line. Upon cloning and sequence analysis of the gene encoding the 24kD protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27
SpecificityDetects a Hsp27 protein, in FACS analysis in samples from human and monkey origins. It does not exhibit reactivity with rodent Hsp25, alphaA, alphaB or beta-crystallin proteins.
Species CrossreactivityHuman and monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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