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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 40 (HSP40, HDJ1)

Anti -Heat Shock Protein 40 (HSP40, HDJ1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP
HSP40, also known as HDJ1 (9), is a basic mammalian 40kD heat shock protein which is homologous to the bacterial heat shock protein, DnaJ, and yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (1,2,3,4). Like many heat shock proteins, HSP40 is inducible by heat, transient metals and azetidine carboxylic acid in mammalian and avian cells (2). In heat shocked HeLa cells, HSP40 translocates rapidly from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (1). This suggests that HSP40 co-localizes with HSP70 and together they repair denatured proteins in the nuclei and nucleoli of heat shocked cells (1). This is analogous to the functions of bacterial DnaJ and DnaK which have been shown to act together in dissociating the bacteriophage lambda protein complexes (5,6) and monomerizing the Rep A dimer (7).
Catalog #H1834-15
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000 (Colorimetric)
Immunoprecipitation: 1:50
Optimal dilutions to be determined by researcher.
Positive ControlsRecombinant Human Hsp40 Protein
HeLa Heat Shocked Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 40% glycerol.
PuritySerum
ImmunogenRecombinant human HSP40
SpecificityDetects a 40kD protein, corresponding to the apparent molecular mass of HSP40 on SDS-PAGE immunoblots. It does not detect DnaJ in E. coli lysates. Species Crossreactivity: Human, monkey, mouse, rat, hamster, guinea pig, rabbit, canine, bovine, sheep, porcine, chicken and fish.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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