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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 40 (HSP40, HDJ1)

Anti -Heat Shock Protein 40 (HSP40, HDJ1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Human HSP40 (heat shock protein with molecular size of 40kD) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK.
Hybridoma:
Mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp40 protein.
Catalog #H1834-15F
Source Ascites
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blotting: 1:1000-1:2000. Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE,
transferred to NC membrane and probed with anti-human Hsp40 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50?. Freeze at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7i2
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human Hsp40(1-340aa) purified from E. coli
SpecificityRecognizes human Heat Shock Protein 40 (HSP40, HDJ1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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