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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -HSP40 (DnaJ Homolog Subfamily B Member 1, Heat Shock 40kD Protein 1, Heat Shock Protein 40, DnaJ Protein Homolog 1, Human DnaJ Protein 1, hDj-1, DNAJB1, DNAJ1, HDJ1, HSPF1)

Anti -HSP40 (DnaJ Homolog Subfamily B Member 1, Heat Shock 40kD Protein 1, Heat Shock
Protein 40, DnaJ Protein Homolog 1, Human DnaJ Protein 1, hDj-1, DNAJB1, DNAJ1, HDJ1,
HSPF1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1, is a basic mammalian 40kD heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1. Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK.
Catalog #H1834-15L
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:10,000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant corresponding to human Hsp40.
SpecificityRecognizes human Hsp40. Species sequence homology: Beluga, bovine, chicken, canine, fish, xenopus, guinea pig, hamster, monkey, mouse, mussel, porcine, rabbit, rat, sheep, scallop.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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