Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -Heat Shock Protein 47 (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47, arthritis-related antigen RA-A47, SERPINH2)

Anti -Heat Shock Protein 47 (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding
protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47,
arthritis-related antigen RA-A47, SERPINH2)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
HSP47, also known as heat shock protein 47, colligin, or gp46, is a collagen-specific stress glycoprotein which resides in the ER and has been shown to be involved in the synthesis/assembly of various collagens as a molecular chaperone. The protein belongs to the serpin (serine protease inhibitor) superfamily but unlike other serpin family proteins, it localizes within the endoplasmic reticulum (ER) because of the presence of RDEL (Arg-Asp-Glu-Leu) sequence, a variant of KDEL sequence, at the C-terminus. HSP47 has been shown to bind to the KDEL receptor in the ER. HSP47 is susceptible to phosphorylation. Both gp46 and HSP47 were later found to be heat-inducible and susceptible to phosphorylation. It is generally believed that HSP47 binds procollagen immediately after it co-translationally enters the ER, and it dissociates from procollagen before its secretion. When cells are heat shocked or treated with inhibitors of the triple-helix formation of procollagen, HSP47 is retained to these abnormal or denatured procollagens for much longer periods of time. Expression of HSP47 on cell surface following alkalization of the endosomal compartments under conditions of stress suggest HSP47 may be a serpin family protein that may modulate cell migration during development and invasion and metastasis in cancer. HSP47 has been suggested to play an important role in tumor metastasis and can be used as a prognostic marker for the metastatic activity of human tumor cells.
Catalog #H1834-20
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000 using ECL.
Immunohistochemistry: 1:50
Optimal dilutions to be determined by researcher.
Recommended Positive Controls:
HeLa Heat Shocked Cell Lysate
Recombinant Rat HSP47 Protein
Human Breast Cancer Tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No4E122
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenNative rat Hsp47 protein
SpecificityRecognizes rat HSP47 at ~47kD. Species Crossreactivity: human, monkey, mouse, rabbit, hamster, guinea pig, bovine, canine, sheep, porcine and chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links