Anti -Heat Shock Protein 47 (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding
protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47,
arthritis-related antigen RA-A47, SERPINH2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH |
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| HSP47, also known as heat shock protein 47, colligin, or gp46, is a collagen-specific stress glycoprotein which resides in the ER and has been shown to be involved in the synthesis/assembly of various collagens as a molecular chaperone. The protein belongs to the serpin (serine protease inhibitor) superfamily but unlike other serpin family proteins, it localizes within the endoplasmic reticulum | | | (ER) because of the presence of RDEL (Arg-Asp-Glu-Leu) sequence, a variant of KDEL sequence, at the C-terminus. HSP47 has been shown to bind to the KDEL receptor in the ER. HSP47 is susceptible to phosphorylation. Both gp46 and HSP47 were later found to be heat-inducible and susceptible to phosphorylation. It is generally believed that HSP47 binds procollagen immediately after it co-translationally enters the ER, and it dissociates from procollagen before its secretion. When cells are heat shocked or treated with inhibitors of the triple-helix formation of procollagen, HSP47 is retained to these abnormal or denatured procollagens for much longer periods of time. Expression of HSP47 on cell surface following alkalization of the endosomal compartments under conditions of stress suggest HSP47 may be a serpin family protein that may modulate cell migration during development and invasion and metastasis in cancer. HSP47 has been suggested to play an important role in tumor metastasis and can be used as a prognostic marker for the metastatic activity of human tumor cells. | | | Catalog # | H1834-20 | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: Colorimetric | | Immunohistochemistry: 1:50 | | Optimal dilutions to be determined by researcher. | | | | Positive Controls: | | HeLa Heat Shocked Cell Lysate | | Recombinant Rat HSP47 (Colligin) Protein | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 4E122 | | Host | Mouse | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Native rat Hsp47 (Colligin) protein. | | Specificity | Recognizes rat HSP47 at ~47kD. Species Crossreactivity: human, monkey, mouse, rabbit, hamster, guinea pig, bovine, canine, sheep, porcine and chicken | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -Heat Shock Protein 47 (HSP47, Colligin, gp46, AsTP3, CBP1, CBP2, Collagen-binding protein 1, Collagen-binding protein 2 precursor, Colligin 1, Colligin 2, gp46, HSP47, arthritis-related antigen RA-A47, SERPINH2) |
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