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You are here:Home » Molecular Biology » Cells-Extracts, Lysates » HeLa Nuclear Extract

HeLa Nuclear Extract

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Specifications

Nuclear extract is prepared from human HeLa cells using a modified protocol of Dignam et al., which contains a variety of DNA-binding proteins and transcription factors.
Catalog #H1838
ApplicationsSuitable for use as a positive control for antibodies.
Recommended UsageAdd an equal volume of Laemmli reducing sample buffer to 5-10ul of lysate. Boil for 5 minutes to reduce the preparation. Load 5-10ul (10-20ug) of reduced lysate per lane for immunoblot analysis.
Storage and StabilityFor long-term storage, store at -70°C. Aliquots are stable for 6 months after receipt at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration~2mg/ml
FormSupplied as a liquid in RIPA buffer containing inhibitors.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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