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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Hemagglutinin Tag (YPYDVPDYA) (HA Tag)

Anti -Hemagglutinin Tag (YPYDVPDYA) (HA Tag)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified E B IH
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteinís biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Catalog #H1847-51
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000
Immunohistochemistry: 1:500
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgY
HostChicken
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to the sequence YPYDVPDYA derived from the influenza virus hemaglutinin (HA) (KLH).
SpecificityRecognizes recombinant proteins containing the HA epitope tag: YPYDVPDYA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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