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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

Hemoglobin is composed of one globin plus four hemes; heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520; however, ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight.
Catalog #	H1850-01B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Capture ELISA: 5ug/ml
	Western Blot: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.2, 0.095% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Human Hemoglobin.
Specificity	Recognizes human Hemoglobin. Forms a single precipitin band with human adult hemoglobin A1, A2, F and S, by immunoelectrophoresis.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.