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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH IC

Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues.The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.
Catalog #	H1850-08D
Applications	Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	ELISA: 1:10,000-1:100,000
	Western Blot: 1:100-1:30,000
	Immunohistochemistry: Paraffin sections
	Immunocytochemistry: 1:200-1:500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.1% Pro-Clean 400. Labeled with Horseradish Peroxidase (HRP).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Hemoglobin A1, A2, F and S
Specificity	Recognizes Hemoglobin. Reacts specifically with human Hemoglobin A1, A2, F and S. No reaction with human serum proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.