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Specifications

Hemoglobin is composed of one globin plus four hemes. Heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520. Ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight.
Catalog #	H1850-15
Contaminants	No reaction against any glycohemoglobins, including HbA1a, HbA1b, HbF and HbAo.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Storage and Stability	For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source	Human erythrocyte lysates
Purity	~ 80% (HPLC)
Form	Supplied as a frozen liquid in 1mM KCN buffer, pH 7.5. One glyco group per -chain monomer N-terminal valine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.