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Specifications

Hemoglobin is composed of one globin plus four hemes. Heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520. Ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight.
Catalog #	H1850-15A
Source
	Hemoglobin A1c from human erythrocytes.
Applications	Suitable for use in ELISA. Other applications not tested
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source	Human erythrocytes
Purity	94% HbA1c by FPLC/Mono S HbA1c column.
Concentration	~5mg/ml (OD415nm)
Form	Supplied as a liquid in 0.05M sodium phosphate, pH 8.0. No preservative added.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.