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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH IC FC IF

Catalog #	H1850-19B
Applications	Suitable for use in Direct Immunofluorescence microscopy, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H326
Host	Mouse
Source	Human
Concentration	~0.6mg/ml
Form	Supplied as a liquid, before the addition of glycerol to 40%. Labeled with FITC.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Purified human fetal hemoglobin
Specificity	Recognizes human fetal hemoglobin. Reactivity is seen with human fetal erythrocytes, adult F cells and nucleated red cell precursors containing HbF. Does not crossreact with other forms of human hemoglobin, including HbA, HBA2, HBA1C, HbS and HbE. Binding is not observed in ELISA assays for purified fetal hemoglobin alpha or gamma globulin chains alone, indicating the binding epitope is present on the intact HbF molecule only. Clone 3H326 binding is at a different epitope on fetal hemoglobin from clone 3H327. They have similar binding affinity as measured by Flow Cytometry and ELISA methods.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.