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Specifications

Heparan sulfate, bovine, is specially prepared from bovine kidney, purified by a combination of chromatographic and chondroitinase ABC treatment.
Catalog #	H1890-55
	Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4)ß-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS-(1->4)-ß-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4)alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc(1->4)-ß-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epimerization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are always found in proximity to N-sulfates which enhances the clustering of the sulfate residues and the heterogeneity in chemical composition and charge density of heparan sulfate.
Purity (TLC)	Single band
Appearance	White powder
Total Nitrogen	2.6-3.2%
Total Sulfur	4.5–6.0%
Inorganic Sulfur	Not detected
Chlorine	Not detected
Uronic Acid (Carbazol reaction)	36.0-40.0%
Glucosamine	30.0–35.0%
Galactosamine	0.01%
Specific Rotation	+75° to +88°
Alternate names	Bovine Kidney; Heparitin Sulfate; Heparin Monosulfate; N?Acetylheparan Sulfate