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Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)
Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH |
|
| Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450kD and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability charateristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling. | | | Catalog # | H1890-90A | | Applications | Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | ELISA: 1:8000 | | Western Blot: 1:100 | | Immunohistochemistry: 1:100; Frozen PLP-fixed sections of bovine and human tissues. | | Optimal dilutions to be determined by the researcher. | | Culture Medium | RPMI 1640 with 10% fetal calf serum. | | Hybridoma | Sp2/O myeloma cells with spleen cells from BALB/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 6F165 | | Host | Mouse | | Source | Bovine | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. No stabilizing proteins added. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Bovine corneal endothelial cells | | Specificity | Highly specific for bovine perlecan. The epitope is located in domain I. No crossreactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species Crossreactivity: Human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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