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Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)
Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH |
|
| Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450kD and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability charateristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (2). | | | Catalog # | H1890-90C | | Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:4000 | | Immunohistochemistry (Frozen bovine, human PLP-fixed sections): 1:50 | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. | | Culture Medium | RPMI 1640 with 10% fetal calf serum. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 6F167 | | Host | Mouse | | Source | Bovine | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 0.01M PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Lysed bovine corneal endothelial cells and extracellular matrix. | | Specificity | Recognizes bovine perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species Crossreactivity: human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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