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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)

Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450kD and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability charateristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (2).
Catalog #H1890-90C
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:4000
Immunohistochemistry (Frozen bovine, human PLP-fixed sections): 1:50
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Culture MediumRPMI 1640 with 10% fetal calf serum.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6F167
HostMouse
SourceBovine
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.01M PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenLysed bovine corneal endothelial cells and extracellular matrix.
SpecificityRecognizes bovine perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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