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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Heparan Sulfate Proteoglycan, Domain 4 (Perlecan, HSPG)

Anti -Heparan Sulfate Proteoglycan, Domain 4 (Perlecan, HSPG)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IC
Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450kD and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability charateristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling (2).
Catalog #H1890-91
ApplicationsSuitable for use in Immunocytochemistry. Other applications have not been tested.
Recommended DilutionImmunocytochemistry: 10ug/ml on primary cultures of hippocampal neurons, fixed with 4% paraformaldehyde in phosphate buffer containing 4% sucrose. The neurons were subsequently permeabilized with 0.25% Triton X-100 for 5 minutes at RT. The fixed and permeabilized cells were blocked with normal donkey serum in Tris buffered saline (TBS) containing 10% Tween. The primary antibody was detected using a FITC-conjugated goat anti-rat secondary antibody.
Optimal dilutions to be determined by the researcher.
HybridomaMouse-rat hybridoma A7L6 (X63.Ag.8 653 mouse myeloma x Fisher rat splenocytes). Propagated in cell culture.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No0.T.58 (A7L6)
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15 sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G chromatography
ImmunogenMurine EHS laminin preparation containing entactin and heparan sulfate proteoglycan.
SpecificityRecognizes mouse low density basement membrane heparan sulfate proteoglycan (perlecan) core protein. Does not crossreact with other basement membrane proteins or fibronectin.
Species CrossreactivityHuman and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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