Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E B IH |
|
| Human perlecan (HSPG2) core protein is a large, complex, multidomain heparan sulfate proteoglycan with a MW of ~467kD (determined from the cDNA sequence). Post-translational modifications of HSPG2 can increase the MW to ~850kD. Perlecan is comprised of five domains. Domain I (N terminal) is predicted to be the site of heparan sulfate linkage to 3 glycosaminoglycans. Domain II is homologous to the LDL receptor. Domain III shares homology with the short arm of laminin A and B chains. Domain IV contains IgG-like repeats similar to those found in N-CAM and has a hydrophobic region suitable for interacting with the membrane. Domain V has regions similar to EGF and the G-domain of laminin A chain. In skin, perlecan is synthesized exclusively by the connective tissue in the dermal layer. Perlecan has been detected in many human tissues, including; breast, colon, liver, lymph nodes, pancreas, and prostate, among others. Proteins similar to perlecan have been identified in colon carcinoma, EHS tumor, and fibrosarcoma cells. | | | Catalog # | H1890-93 | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-2ug/ml | | Immunohistochemistry (Frozen or FFPE): 5-10ug/ml. Note: Formalin-fixed, paraffin sections require pretreatment with 0.1% protease for 30 minutes at RT prior to the blocking step. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Human breast, colon, liver, lymph nodes, pancreas and prostate tissues. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 3G166 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. | | Purity | Purified | | Immunogen | Recombinant protein corresponding to Perlecan domain III. | | Specificity | Recognizes domain III of human Heparan Sulfate Proteoglycan. Domain III shares homology with the short arm of laminin A and B chains. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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