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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)

Anti -Heparan Sulfate Proteoglycan (Perlecan, HSPG)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
Human perlecan (HSPG2) core protein is a large, complex, multidomain heparan sulfate proteoglycan with a MW of ~467kD (determined from the cDNA sequence). Post-translational modifications of HSPG2 can increase the MW to ~850kD. Perlecan is comprised of five domains. Domain I (N terminal) is predicted to be the site of heparan sulfate linkage to 3 glycosaminoglycans. Domain II is homologous to the LDL receptor. Domain III shares homology with the short arm of laminin A and B chains. Domain IV contains IgG-like repeats similar to those found in N-CAM and has a hydrophobic region suitable for interacting with the membrane. Domain V has regions similar to EGF and the G-domain of laminin A chain. In skin, perlecan is synthesized exclusively by the connective tissue in the dermal layer. Perlecan has been detected in many human tissues, including; breast, colon, liver, lymph nodes, pancreas, and prostate, among others. Proteins similar to perlecan have been identified in colon carcinoma, EHS tumor, and fibrosarcoma cells.
Catalog #H1890-93
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml
Immunohistochemistry (Frozen or FFPE): 5-10ug/ml. Note: Formalin-fixed, paraffin sections require pretreatment with 0.1% protease for 30 minutes at RT prior to the blocking step.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman breast, colon, liver, lymph nodes, pancreas and prostate tissues.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G166
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified
ImmunogenRecombinant protein corresponding to Perlecan domain III.
SpecificityRecognizes domain III of human Heparan Sulfate Proteoglycan. Domain III shares homology with the short arm of laminin A and B chains.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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