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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Heparan Sulfate Proteoglycan (HSPG)

Anti -Heparan Sulfate Proteoglycan (HSPG)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200kD (aorta) and 30-160kD (kidney) and a core protein size of 24kD or 22kD, respectively, was localized by immunohisto-hemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
Catalog #H1890-93A
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: < 1ug/ml
Western Blot: 1ug/ml
Immunohistochemistry: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No1F10/B8
HostMouse
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from 50mM Tris, pH 7.4. Reconstitute with 10ul sterile ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human small basement membrane heparan sulfate proteoglycan (HS-PG)
SpecificityRecognizes human Heparan-Sulfate-Proteoglycan. Specific for a core protein epitope of a human small basement membrane heparan sulfate proteo-glycan (HS-PG).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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