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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Heparan-Sulfate Proteoglycan

Anti -Heparan-Sulfate Proteoglycan

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
The principal molecular structure of basement membranes has been elucidated during the past two decades and it was shown that for example in the glomerular basement membrane (GBM) HS-PG are responsible for the selective filtration process. Removal of HS-PG will lead to proteinuria. Whereas previously perlecan was the only known basement membrane HS-PG, there is now evidence that (at least two) other basement membrane HS-PG do exist: Agrin, originally discovered as an important component of the neuromuscular junction and a novel small HS-PG, that was isolated from human aorta and kidney. This HS-PG, with a molecular weight of 80-200kD (aorta) and 30-160kD (kidney) and a core protein size of 24kD or 22kD, respectively, was localized by immunohisto-hemistry to the basement membrane. Amino acid sequence analysis of tryptic peptides indicate, that this small HS-PG is clearly distinct from perlecan and agrin.
Catalog #H1890-93B
ApplicationsSuitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1ug/ml
Western Blotting: 1ug/ml
Immunohistochemistry: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2H7/G11
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 50mM TBS, pH 7.2.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human small basement membrane heparan sulfate proteoglycan (HS-PG)
SpecificityRecognizes human Heparan-Sulfate-Proteoglycan. specific for a core protein epitope of a human small basement membrane heparan sulfate proteo-glycan (HS-PG).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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