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You are here:Home » Antibodies » Abs to Enzymes, Heparanase » Anti -Heparanase 1 (HEP1)

Anti -Heparanase 1 (HEP1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.
Catalog #H1890-95
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:4000. Using lysate from 2x10e4 transfected CHO cells. 4-12% SDS-PAGE gel used; proteins were transferred to a PVDF membrane.
Immunohistochemistry: 1:40
Immunocytochemistry: 10ug/ml stained neutrophils and platelets from human blood smears.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No5C41
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% thimerosal.
PurityPurified by Protein G affinity chromatography.
ImmunogenPolypeptide from the 50kD subunit of Heparanase
SpecificityRecognizes the 50kD subunit and the 65kD precursor of human Heparanase. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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