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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E

Heparin Cofactor II (HCII) is a single chain glycoprotein produced in the liver and circulates in plasma at concentration of 50-100ug/ml (0.8-1.5uM). A member of the SERPIN family of proteinase inhibitors, HCII exhibits a relatively narrow specificity and is only known to inhibit the serine proteinases thrombin and chymotrypsin. The inhibitory activity toward thrombin is accelerated up to 1000-fold in the presence of optimal concentrations of heparin. Unlike Antithrombin, thrombin inhibition by HCII is also enhanced by dermatan sulphate. HCII has an apparent molecular weight of 65,600 daltons and interaction with thrombin results in a covalent 1:1 enzyme-inhibitor complex of 102,600 daltons (1-3).
Catalog #	H1892-51B
Applications	Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Rz Ratio (Reinheitszahl, A403/A280)	0.46
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Purity	Purified by immunoaffinity chromatography.
Immunogen	Heparin Cofactor II isolated from human plasma.
Specificity	Recognizes human Heparin Cofactor II.
Species Crossreactivity	Canine, mouse, porcine, rabbit, rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.