| Catalog # | H1905-13 |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:100-1:500 |
| Immunohistochemistry (Frozen and paraffin): 1:100; Paraffin sections do not require protein digestion pre-treatment or antigen retrieval. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Postive Control Tissue | Liver carcinoma/Hepatitis B infected liver |
| Recommoned Secondary Antibodies | I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt x Mo |
| I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb x Mo |
| Recommended Negative Control | I1904-83P1: IgG2a Murine Negative Control |
| Hybridoma | Spleen cells from immunized BALB/c mice were fused with cells of a mouse myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3H2188 |
| Host | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | HBxAg-Protein A Fusion protein |
| Specificity | Recognizes Hepatitis B X Antigen (17kD). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
