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You are here:Home » Kits and Assays » Kits for ELISA (Infectious Disease), BioAssay™ » Anti-Hepatitis B Virus E Antigen (anti-HBeAg) ELISA Kit, BioAssay™ (Human)

Anti-Hepatitis B Virus E Antigen (anti-HBeAg) ELISA Kit, BioAssay™ (Human)

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Specifications

The Anti-Hepatitis B Virus E Antigen (HBeAg) ELISA Kit is a qualitative in vitro enzyme immunoassay for the detection of anti-HBe in human serum or plasma.
Catalog #H1910-40J
Principle of the AssayThe purified Anti-HBe is coated on the solid phase of multi-wells. Serum sample, recombinant HBeAg and horseradish peroxidase labeled with Anti-HBe (conjugated) are added to coated wells, and form a competitive combination. After incubation, if Anti-HBe content is high in the sample, a complex of HBeAg-Anti-HBe-Anti-HBe labeled with HRP will form. The wells are washed to remove the complex, and incubated with substrate (TMB), subsequently producing no color change or little color change. If Anti-HBe is not present in the sample, there will be significant color change. The test is sensitive, reproducible and easy to operate.
Kit ComponentsH1910-40J1: Microtiter Plate, 1x96wells
H1910-40J2: Enzyme Conjugate, 1x6ml
H1910-40J3: Positive Control Serum, 1x500ul
H1910-40J4: Negative Control Serum, 1x500ul
H1910-40J5: Wash Buffer (20X), 1x30ml
H1910-40J6: Substrate A, 1x6ml
H1910-40J7: Substrate B, 1x6ml
H1910-40J8: Stop Solution, 1x6ml
H1910-40J9: Neutralization Reagent, 1x6ml
Interpretation of ResultsCut-off Value (COV) Calculation:
COV = average OD450 of negative controls X 0.5
Positive OD450 of sample <COV
Negative: OD450 of sample COV
Invalid: If the OD of negative control is below or equal to 1.000, the result is invalid. In any event, repeat the test. If the problem persists, contact technical support.
Performance CharacteristicsSensitivity: 4NCU/ml (per Biological Reference Reagents of Chinese Clinical Test Center), OD 0.600
Specificity: The average OD of 20 normal negative smples is 2.00
Precision: %CV 20% (n=10)
Storage and StabilityStore all kit components at 4°C. Kit is stable for 6 months.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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