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You are here:Home » Antibodies » Abs to Infectious Disease Hepatitis » Anti -Hepatitis B Core Antigen (HBcAg)

Anti -Hepatitis B Core Antigen (HBcAg)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E IH
Hepatitis B virus is spherical in shape with a diameter of 42nm. It contains a 27nm partially double stranded DNA core enclosed within a lipoprotein coat. The antigenic activity of the nucleocapsid core is designated as hepatitis B core antigen. The antigens in the outer surface are called as hepatitis B virus surface antigens. Core antigens are localized within the nuclei whereas the surface antigens are present in the cytoplasm of the infected cells. Antibodies to surface antigens appear in circulation at an early stage of infection whereas the antibodies to the core antigens are detected after several weeks.
Catalog #H1920-10D
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: (Formalin/paraffin): 1:50-1:100 for 30 min at RT. No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilution to be determined by researcher.
Positive ControlHepatitis B virus infected liver
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide, before the addition of glycerol to 40%.
PurityPurified antibody fraction from rabbit anti-serum.
ImmunogenHepatitis B virus.
Cellular LocalizationNuclear
SpecificityRecognizes a protein in the core of hepatitis B virus. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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