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You are here:Home » Antibodies » Abs to Infectious Disease Hepatitis » Anti -Hepatitis C Virus Core Protein (HCV)

Anti -Hepatitis C Virus Core Protein (HCV)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E
Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission.   HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of Core Protein (Capsid Protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6kB HCV genome has a single open-reading frame, which is to be translated into a single polyprotein.  HCV viral proteins are produced after processing the polyprotein.  Genes for core protein and envelop proteins are located adjacently at the 5-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.
Catalog #H1920-14A
ApplicationsSuitable for use in ELISA. Not suitable for Western Blot. Other applications not tested.
Recommended DilutionsELISA (Indirect): The testing plates were coated with HCV non-structural protein (NS), core protein (C) and the mixture of NS and C respectively. The neat culture supernatant of hybridoma showed a strong reaction with HCV capsid protein (OD492 value >2) and with mixture of NS and C (OD492 value >1.5). Showed no reaction with HCV NS (OD492 value <0.03).
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0-AG14 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityStorage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3H149
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptides derived from HCV core protein
SpecificityRecognizes Hepatitis C Virus Core Protein. Reactive to recombinant core protein C + envelope protein M (aa1-142 on HCV polyprotein) and synthetic core protein C (aa1-61 on HCV polyprotein). Does not react with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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