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You are here:Home » Antibodies » Abs to Infectious Disease Hepatitis » Anti -Hepatitis C Virus Core Protein (HCV)

Anti -Hepatitis C Virus Core Protein (HCV)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #H1920-14A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: Indirect ELISA method. The testing plates were coated with HCV Non-Structural Protein (NS), Capsid Protein (C) and the mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV C (OD492 value > 2) and mixture of NS and C (OD492 value >1.5). Showed no reaction with HCV NS (OD492 value < 0.03).
Western Blot 0.5-1ug/ml will allow visualization 0.1ug/lane (Recombinant CPC+EPM (core)), 0.5ug/lane (Synthetic CPC), and 0.1ug/lane (Recombinant Chimeric HCV Polyprotein). Testing is under reducing and non-reducing conditions.
Optimal dilutions to be determined by the researcher.
MyelomaSp2/0-Ag14
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3H149
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 40% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptides derived from HCV capsid protein
SpecificityReactive with Recombinant Capsid Protein C and Envelop Protein M (core) (1-142aa) and Synthetic Capsid Protein C (1-61aa). Recognizes different antigenic determinants of HCV capsid protein. Does not crossreact with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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