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You are here:Home » Antibodies » Abs to Infectious Disease Hepatitis » Anti -Hepatitis C Virus (HCV) NS-3 Region (aa1350-1460)

Anti -Hepatitis C Virus (HCV) NS-3 Region (aa1350-1460)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000aa. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70kD.
Catalog #H1920-22G2
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested.
Recommended DilutionELISA: ~10-6
Immunohistochemistry: 1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9L330
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant antigen (HCV genotype 1b).
SpecificityRecognizes HCV NS-3 Antigen (aa1350-1460).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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