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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Catalog #	H1920-22P
Applications	Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 0.1-1ug/ml will allow visualization of 100ng/lane of both Recombinant Chimeric HCV Polyprotein and Recombinant NS-3 Protein. No reaction can be seen with Synthetic NS-3. Testing is under reducing and non-reducing conditions.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3H156
Host	Mouse
Concentration	~1mg/ml
Form	Lyophilized from 0.01M PBS, pH 7.2.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified Recombinant Chimeric HCV Polyprotein (555 a.a)
Specificity	Recombinant NS-3 (1252aa-1477aa), Synthetic NS-3 (1378aa-1458aa) and Recombinant Chimeric HCV Polyprotein (60kD.). No crossreaction can be seen with Recombinant CPC* + EPM** (core) (1aa-142aa), Synthetic CPC* (1aa-61aa) and Synthetic NS-4a Protein (1689aa-1735aa).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.