Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-1ug/ml will allow visualization of 100ng/lane of both Recombinant Chimeric HCV Polyprotein and Recombinant NS-3 under reducing and non-reducing conditions. Optimal dilution determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified Recombinant Chimeric HCV Polyprotein (555aa)
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recombinant NS-3 (aa1252-1477aa) and Recombinant Chimeric HCV Polyprotein (60kD.). No crossreaction can be seen with Recombinant CPC* + EPM** (core) (aa1-142), Synthetic CPC* (aa1-61), Synthetic NS-3 (aa1378-1458) and Synthetic NS-4a Protein (aa1689-1735).
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