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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Catalog #	H1920-22S
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 0.1-1ug/ml will allow visualization of 100ng/lane of both Recombinant Chimeric HCV Polyprotein and Recombinant NS-3 under reducing and non-reducing conditions.
	Optimal dilution determined by the researcher.
Hybridoma	Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3H159
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 40% glycerol.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Purified Recombinant Chimeric HCV Polyprotein (555 a.a)
Specificity	Recombinant NS-3 (aa1252-1477aa) and Recombinant Chimeric HCV Polyprotein (60kD.). No crossreaction can be seen with Recombinant CPC* + EPM** (core) (aa1-142), Synthetic CPC* (aa1-61), Synthetic NS-3 (aa1378-1458) and Synthetic NS-4a Protein (aa1689-1735).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.