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You are here:Home » Molecular Biology » Growth Factors-HCC » Hepatocellular Carcinoma, Recombinant, Human (Hemofiltrate Carcinoma Chemokine, HCC-1)

Hepatocellular Carcinoma, Recombinant, Human (Hemofiltrate Carcinoma Chemokine, HCC-1)

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Specifications

Human Hemofiltrate CC chemokine-1 (HCC-1) was first isolated from the hemofiltrate of patients with chronic renal failure. HCC-1 has a 46% amino acid sequence identity with MIP-1a/MIP-1b and 29-37% homology with other CC chemokines. Recombinant Human HCC-1 is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8411D.
Catalog #H1999-02
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Thr-Glu-Ser-Ser-Ser.
Dimers and Aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityHCC-1 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract Human monocytes at 5.0-20.0ng/ml.
Endotoxin 0.1ng/ug (IEU/ug) of Human HCC-1.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of HCC-1 as a Reference Standard.
ReconstitutionReconstitute the lyophilized Human HCC-1 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. No additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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