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Specifications

Catalog #	H2004-70A2
Description	Hepatocyte culture mdium was designed and tested for our rat hepatocyte. This is the Hepatocyte culture medium supplement component for H2004-70A. The hepatocyte culture medium is a serum-free medium supplemented with BSA and several recombinant growth factors.
	Each lot of this product is tested in cell culture to ensure its sterility. This product does not contain antibiotics.
Use of Hepatocyte Culture Medium for Culturing Rat Hapatocytes	1. Thaw the supplement and add the entire volume (5ml) to the basal medium (500ml). Antibiotics may also be added if contamination is a concern.
	2. Take the amount of hepatocyte culture medium needed and warm to 37°C in a water bath or incubator. Avoid frequent temperature change to the entire bottle of medium. The hepatocyte culture medium is stable for at least 1 month stored at 4°C.
Storage and Stability	The hepatocyte culture medium supplement is stored at 4°C and is stable for at least 6 months.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.