| Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro. It may modulate hematopoiesis. |
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| Recombinant Human HGF is a heterodimer, non-glycosylated, polypeptide chain consisting an alpha-chain of 463aa and beta-chain of 234aa having a total molecular mass of 80kD. |
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| Catalog # | H2005-02 |
| Sequence | Agrees with the seqeunce of native human HGF. |
| Endotoxin | 0.1ng/ug (IEU/ug) of HGF. |
| Dimers and Aggregates | < 1% as determined by silver-stained SDS-PAGE gel analysis. |
| Biological Activity | Hepatocyte Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells, was found to be 20-40ng/ml. |
| Protein Content | Protein quantitation was carried out by two independent methods: |
| 1. UV spectroscopy at 280nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| 2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 24 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source | Human, recombinant protein expressed by Baculovirus Infected High-5 cells |
| Purity | 98% by by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug). |
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| Form | Supplied as a lyophilized powder. Reconstitute in sterile dH2O, 0.1% HSA or BSA to a concentration > 0.1mg/ml. Can be further diluted to other aqueous solutions. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
