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You are here:Home » Molecular Biology » Growth Factors-HGF » Hepatocyte Growth Factor, Recombinant, Human (HGF, Scatter Factor, SF, Hepatopoietin A)

Hepatocyte Growth Factor, Recombinant, Human (HGF, Scatter Factor, SF, Hepatopoietin A)

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Specifications

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro. It may modulate hematopoiesis.
Recombinant Human HGF is a heterodimer, non-glycosylated, polypeptide chain consisting an alpha-chain of 463aa and beta-chain of 234aa having a total molecular mass of 80kD.
Catalog #H2005-02
SequenceAgrees with the seqeunce of native human HGF.
Endotoxin 0.1ng/ug (IEU/ug) of HGF.
Dimers and Aggregates< 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityHepatocyte Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells, was found to be 20-40ng/ml.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 24 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman, recombinant protein expressed by Baculovirus Infected High-5 cells
Purity98% by by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug).
FormSupplied as a lyophilized powder. Reconstitute in sterile dH2O, 0.1% HSA or BSA to a concentration > 0.1mg/ml. Can be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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