Anti -Hepcidin, CT, 13aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed
Antimicrobial Peptide)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
E B |
|
| Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, pro-protein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS). | | | Catalog # | H2008-53 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilutions | ELISA: ~1:100,000 using 50-100ng control peptide/well. | | Western Blot: 1:1000-1:5000 (ECL). | | Optimal dilutions to be determined by the researcher. | | Control Peptide | H2008-55: Hepcidin, Mouse, 13aa, CT, Control Peptide | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Mouse | | Concentration | Not Determined | | Form | Supplied as a liquid in PBS, 0.05% sodium azide. | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to 13aa from mouse HEPC. Species Sequence Homology: rat, 92%; human, 69% (20-, 22- and 25aa C-terminal fragments) | | Specificity | Recognizes mouse Hepcidin. May react with the unprocessed larger 60aa pro-HEPC if present in samples or tissues. | | Epitope | ~C-terminal of mature HEPC | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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