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You are here:Home » Antibodies » Antibodies-Peptides » Anti -Hepcidin, pro (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Anti -Hepcidin, pro (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed
Antimicrobial Peptide)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The signal peptide is cleaved to produce pro-HEPC, which is further processed to generate the mature secreted form of hepcidin (C-terminal 20, 22 or 25-aa HEPC). In humans, 20-aa and 25-aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus.
The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is up-regulated by lipopolysaccharides (LPS).
Catalog #H2009-02A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:100,000 using 50-100ng control peptide
H2009-02B per well.
Western Blot: 1-10ug/ml using Chemiluminescence.
Optimal dilutions to be determined by the researcher.
Control PeptideH2009-02B Hepcidin, pro, Human, Control Peptide
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, 11-aa, corresponding to the N-terminus of human Pro-HEPC (KLH).
SpecificityRecognizes human Hepcidin, pro (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide). Does not react with the secreted forms of HEPC. Species sequence homology: mouse and rat 63%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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