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You are here:Home » Biochemicals » Biochem-Molecular Biology » HEPES Free Acid

HEPES Free Acid


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Catalog #H2010
HEPES is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good’s buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes.
Appearance White, crystalline powder
Purity 99.0%
Identity (IR) Conform to reference
Solubility Colorless, clear, complete
A260nm (1M) 0.05
A280nm (1M) 0.05
A405nm (1M) 0.05
pH 5.2±0.5
pKa 7.55 ± 0.2
Water (KF) 0.5%
Residue on Ignition 0.5%
Heavy Metals 0.001%
RNase, DNase, Protease None Detected
Endotoxin 1EU/ml
Certificate of Origin China
CAS Number7365-45-9
Molecular FormulaC8H18N2O4S
Molecular Weight238.31
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesN-[2-Hydroxyethyl]piperazine-N?-[2-ethanesulfonic acid]

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