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Specifications

HEPES Buffer, 1M, is prepared from HEPES Free acid with sterile-filtered, Molecular Biology grade water and is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good’s buffer useful in cell culture media formulations.
Catalog #	H2010-01
Selection of biological buffer systems should include the following criteria	exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes.
CAS Number	75277-39-3
Molecular Formula	C8H17N2O4S
Molecular Weight	260.3
Appearance	Colorless, clear liquid
pH	4.60-5.60
Purity	99%
pKa	7.55
Heavy Metals	0.0001%
RNase, DNase, Protease	None Detected
Endotoxin	1Eu/ml
Alternate names	N-[2-Hydroxyethyl]piperazine-N?-[2-ethanesulfonic acid]